In an in Vitro Transcription Assay to Detect the Level $\beta$

question 17

Multiple Choice

In an in vitro transcription assay to detect the level of transcription from a lac operon,one of the RNA precursors was tagged with a fluorescent label on the $\beta$ -phosphate.Which of the following is a likely outcome if transcription occurred?

• A)The level of fluorescence would increase along the new RNA strand.
• B)Each RNA would have a fluorescent tag on every other nucleotide.
• C)The level of fluorescence would be very high in the assay buffer.
• D)No fluorescence would be detected in the buffer at the end of the assay.
• E)All of the choices are possible.

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