An Experiment Was Designed to Obtain Nonspecific Transcription from Both

question 5

Multiple Choice

An experiment was designed to obtain nonspecific transcription from both strands of a DNA molecule.Which of the following strategies would be most effective in achieving this?

• A)Include the RNA holoenzyme in the reaction.
• B)Use the core enzyme of RNA polymerase.
• C)Enrich the preparation with sigma subunit.
• D)Use intact DNA .
• E)Include the RNA holoenzyme in the reaction and use the core enzyme of RNA polymerase are both effective.