A Recombinant Plasmid Was Constructed with Several Restriction Enzyme Sites

question 10

Multiple Choice

A recombinant plasmid was constructed with several restriction enzyme sites and an ampicillin resistance marker.This recombinant was used to transform a host bacteria that also has the ampicillin resistant gene.Which of the following statements is true regarding this experiment?

• A)All transformed bacteria will grow on a plate containing ampicillin.
• B)All untransformed bacteria will grow on a plate containing ampicillin.
• C)It will be difficult to screen for a colony containing a plasmid.
• D)A bacterial host that is ampicillin-sensitive should be used for the screening.
• E)All of the choices are true.

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