A Recombinant Plasmid Was Constructed with Several Restriction Enzyme Sites

question 10

Multiple Choice

A recombinant plasmid was constructed with several restriction enzyme sites and an ampicillin resistance marker.This recombinant was used to transform a host bacteria that also has the ampicillin resistant gene.Which of the following statements is true regarding this experiment?

• A)All transformed bacteria will grow on a plate containing ampicillin.
• B)All untransformed bacteria will grow on a plate containing ampicillin.
• C)It will be difficult to screen for a colony containing a plasmid.
• D)A bacterial host that is ampicillin-sensitive should be used for the screening.
• E)All of the choices are true.

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Definitions:

Skin Wound

A break or opening in the skin that results from injury or surgical incision.

Blood Clot

A mass of blood cells and coagulation factors that form to stop bleeding and facilitate healing.

Malignant Tumor

A cancerous growth capable of invading nearby tissues and spreading to different body parts, posing serious health risks.

Epithelial Tissue

A protective tissue that covers the body surface, lines internal organs, and forms glandular tissue.