One Approach to Prevent Religation of the Ends of a Restricted

question 7

Multiple Choice

One approach to prevent religation of the ends of a restricted plasmid DNA is to

• A)store the plasmid in DNase.
• B)store the plasmid in RNAase.
• C)treat the plasmid with alkaline phosphatase to remove phosphates from the 'sticky ends'
• D)treat the plasmid with a protease to remove all proteins.
• E)treat the plasmid with BamHI to create gaps.

---

Definitions:

Rigidly Segregated

An environment or society where individuals are strictly separated based on certain characteristics, such as race.

CCC

The Civilian Conservation Corps, a public work relief program that operated in the United States for unemployed, unmarried men during the Great Depression, part of the New Deal.

Agricultural Adjustment Administration

A New Deal agency established in 1933 to improve agricultural prices by controlling production, often through paying farmers not to plant on part of their land.

Sharecroppers

Farmers who rent small plots of land to farm and in return, they give a portion of their crop yield to the landlord as rent.