A Recombinant Plasmid Was Constructed with Several Restriction Enzyme Sites

question 10

Multiple Choice

A recombinant plasmid was constructed with several restriction enzyme sites and an ampicillin resistance marker.This recombinant was used to transform a host bacteria that also has the ampicillin resistant gene.Which of the following statements is true regarding this experiment?

• A)All transformed bacteria will grow on a plate containing ampicillin.
• B)All untransformed bacteria will grow on a plate containing ampicillin.
• C)It will be difficult to screen for a colony containing a plasmid.
• D)A bacterial host that is ampicillin-sensitive should be used for the screening.
• E)All of the choices are true.

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Definitions:

African Slaves

People from Africa who were forcibly transported to the New World and sold into slavery, primarily to work on plantations.

Bacon's Rebellion

A 1676 uprising in colonial Virginia, led by Nathaniel Bacon, against the colony's government for its failure to protect settlers from Native American raids.

Virginia

A state located in the Southeastern and Mid-Atlantic regions of the United States, known for its significant contributions to the formation of the nation's history and government.

Puritans

A religious group in the 16th and 17th centuries advocating for purification of the Church of England from Roman Catholic practices.