Polymerase Chain Reaction (PCR) Can Be Used to Perform DNA

question 31

Multiple Choice

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

• A) Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA.
• B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer.
• C) No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs.
• D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process.

Comprehend the accounting implications of promissory notes on accounts payable and receivable.

Understand the process and accounting implications of renewing a note at maturity.

Recognize the accounting treatment for non-payment or default on a promissory note.

Understand the impact of collecting a note receivable on interest income and how it affects the balance sheet.

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Definitions:

Statement of Owner's Equity

A financial statement detailing changes in the owner's equity of a company over a specific period, including contributions, withdrawals, and the net income or loss.

Trial Balance

A bookkeeping worksheet in which the balances of all ledgers are compiled into debit and credit account column totals that are equal.

Adjustments

At the close of an accounting period, records are adjusted to attribute revenues and expenses to the timeframe in which they truly happened.

Adjusted Trial Balance

A list of all accounts and their balances after adjustments have been made, used as the basis for preparing financial statements.