Put the Following Steps in the Correct Order Needed to Produce

question 27

Multiple Choice

Put the following steps in the correct order needed to produce a recombinant vector containing a human gene insert. I. Introduce the recombinant plasmid into a bacterial cell.
II) Isolate the human gene and the vector DNA using restriction enzymes.
III) Ligate the DNA fragments to produce a recombinant plasmid.
IV) Grow bacterial cells on a medium containing a selective agent (such as an antibiotic) .

• A) II, I, III, IV
• B) II, III, I, IV
• C) III, I, II, IV
• D) III, I, IV, II
• E) I, III, IV, II

Identify the factors that influence the thickness of the lithosphere.

Understand how erosion and deposition affect crustal thickness and surface elevation.

Acknowledge the contributions of tectonic settings to regional mountain formation.

Interpret geological evidence for past and present uplift phenomena.