EcoRI Makes Staggered Cuts When It Cleaves DNA,creating Single-Stranded Tails

question 38

Multiple Choice

EcoRI makes staggered cuts when it cleaves DNA,creating single-stranded tails called "sticky ends." These ends can form a complementary base pair.Assuming that the concentration of cut DNA strands is not rate limiting in order for the complementary ends to base pair,which condition is necessary?

• A) The presence of specific helicases
• B) Sufficiently high temperature to dissociate complementary strands
• C) Methyl groups at the end of each DNA strand
• D) Sufficiently low temperature to encourage hydrogen binding of DNA strands
• E) Phosphodiester bonds between the complimentary DNA strands

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