The Following Table Presents the Effect of Different Media on the Growth

question 38

Essay

The following table presents the effect of different media on the growth response of tryptophan mutations in Salmonella typhimurium (+ = growth,- = no growth).
$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\text { Medium. Supplemented with }$

$\begin{array}{l}\begin{array}{lll}\underline{\text { Straint }}\\\text { trp-8 }\\\text {trp-2}\\\text {trp-3}\\\text {trp-1}\\\end{array}\begin{array}{c}\underline {\text { No Supplement} }\\-\\-\\-\\-\end{array}\begin{array}{lll}\text { IGP } & \text { AA } & \text { IN } & \text { TRY } \\\hline+ & + & + & + \\+ & - & + & + \\- & - & + & + \\- & - & - & +\end{array}\end{array}$

(a)Construct the biochemical pathway for the compounds IGP,AA,IN,and TRY based on these data.
(b)Place strains of bacteria (mutations)in the appropriate steps in the pathway.
(c)In bacteria,it is often possible to make partial diploid strains.Assume that a diploid strain was made containing the complete genomes of the trp-2 and trp-1 strains.Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.

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