A Plasmid That Encodes Resistance to Ampicillin and Tetracycline Is

question 96

Short Answer

A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid? (b) What antibiotic-resistance phenotypes will be found on the plate? (c) Which phenotype will indicate the presence of plasmids that contain human DNA fragments?

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Definitions:

Personality Problems

Issues or difficulties in an individual's character traits that cause significant distress or impairment in social, occupational, or other important areas of functioning.

Therapy Moves Slowly

A perspective emphasizing that the process of psychotherapy often requires time to affect meaningful and lasting change in an individual's psychological state.

Patient's Problems

The specific issues or concerns that a patient brings into a therapeutic or medical context for understanding, management, or resolution.

Psychoanalysis

A therapeutic approach originally developed by Sigmund Freud, focusing on unconscious processes as they are manifested in a person's behavior.