In DNA Sequencing by the Sanger (Dideoxy) Method

question 28

Multiple Choice

In DNA sequencing by the Sanger (dideoxy) method:

• A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands.
• B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis.
• C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence.
• D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands.
• E) the template DNA strand is radioactive.

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Definitions:

Task Cohesion

Task cohesion refers to the level of unity and commitment among members of a group or team towards achieving a common goal.

Social Cohesion

The bonds or "glue" that bring people together in society, fostering a sense of belonging and mutual support.

Presumed Competence

The assumption that an individual is capable and knowledgeable in a certain area without needing explicit evidence.

Primary Group

A group with strong emotional ties and bonds that endure over time. This type of group tends to be more informal and intimate than other groups that individuals belong to. See also Secondary group.