The Double-Reciprocal Transformation of the Michaelis-Menten Equation,also Called the Lineweaver-Burk

question 27

Multiple Choice

The double-reciprocal transformation of the Michaelis-Menten equation,also called the Lineweaver-Burk plot,is given by
1/V₀ = K_m /(V_max[S]) + 1/V_max
To determine K_m from a double-reciprocal plot,you would:

• A)multiply the reciprocal of the x-axis intercept by -1.
• B)multiply the reciprocal of the y-axis intercept by -1.
• C)take the reciprocal of the x-axis intercept.
• D)take the reciprocal of the y-axis intercept.
• E)take the x-axis intercept,where V₀ = 1/2 V_max.

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