Experiments on Denaturation and Renaturation After the Reduction and Reoxidation

question 61

Multiple Choice

Experiments on denaturation and renaturation after the reduction and reoxidation of the -S-S- bonds in the enzyme ribonuclease (RNase) have shown that:

• A) folding of denatured RNase into the native, active conformation, requires the input of energy in the form of heat.
• B) native ribonuclease does not have a unique secondary and tertiary structure.
• C) the completely unfolded enzyme, with all -S-S- bonds broken, is still enzymatically active.
• D) the enzyme, dissolved in water, is thermodynamically stable relative to the mixture of amino acids whose residues are contained in RNase.
• E) the primary sequence of RNase is sufficient to determine its specific secondary and tertiary structure.

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Definitions:

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