One Method Used to Prevent Disulfide Bond Interference with Protein

question 81

Multiple Choice

One method used to prevent disulfide bond interference with protein sequencing procedures is:

• A) cleaving proteins with proteases that specifically recognize disulfide bonds.
• B) protecting the disulfide bridge against spontaneous reduction to cysteinyl sulfhydryl groups.
• C) reducing disulfide bridges and preventing their re-formation by further modifying the -SH groups.
• D) removing cystines from protein sequences by proteolytic cleavage.
• E) sequencing proteins that do not contain cysteinyl residues.

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Definitions:

Independent Variable

In research, a variable that is manipulated or changed by the researcher to observe its effects on the dependent variable.

Control

In sociology, control refers to the mechanisms, strategies or institutions that regulate individuals' behavior within a society to maintain order and enforce norms.

Action Research

A type of research aimed at creating social change, in which the researcher works closely with members of a community who participate in the research process and collaborate toward the goal of social change.

Objectivity

The practice of maintaining neutrality and impartiality, avoiding bias and personal judgment, especially in research and reporting.