Why Do Smaller Molecules Elute After Large Molecules When a Mixture

question 100

Short Answer

Why do smaller molecules elute after large molecules when a mixture of proteins is passed through a size-exclusion (gel filtration) column?

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Definitions:

Personality

The combination of characteristics or qualities that form an individual's distinctive character, influencing behavior, thoughts, and emotions.

Pretend

Act as if something is true while knowing it is not, often as a part of play or to deceive.

Appraisal

The process of evaluating the performance, value, or condition of something, typically for the purpose of making decisions based on that assessment.

Discreetly

In a careful, prudent manner, ensuring confidentiality or avoiding unwanted attention.