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You Are Trying to Isolate an Enzyme That Catalyzes the Conversion

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Essay

You are trying to isolate an enzyme that catalyzes the conversion of A \rightarrow B,and you have a sensitive assay for this enzyme.After lysing open the cells,you find that the activity is associated with the membrane fraction,not the soluble fraction (which you throw away).You then follow the procedure shown below.Explain the sudden re-occurrence of activity at the end of your protocol.  You are trying to isolate an enzyme that catalyzes the conversion of A  \rightarrow   B,and you have a sensitive assay for this enzyme.After lysing open the cells,you find that the activity is associated with the membrane fraction,not the soluble fraction (which you throw away).You then follow the procedure shown below.Explain the sudden re-occurrence of activity at the end of your protocol.


Definitions:

Atmospheric Oxygen

The oxygen gas (O2) present in the Earth's atmosphere, essential for the respiratory processes of most living organisms.

Photosynthesizing Bacteria

Microorganisms that convert light energy into chemical energy through the process of photosynthesis.

Terrestrial Plants

Plants that grow on land, as opposed to aquatic (water-based) or epiphytic (growing on other plants) plants.

Primitive Atmosphere

The early atmosphere of Earth, which was very different in composition from the current one, possibly composed of gases such as methane, ammonia, and water vapor.

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