The Principle of Solid Support Hybridization (Often Called Blotting) Is

question 22

Multiple Choice

The principle of solid support hybridization (often called blotting) is:

• A) The target sequence is part of the liquid support, and the probe, which is attached to solid support, hybridizes to the target.
• B) All the target nucleic acids are gathered in one spot on the electrophoresis gel. Then all the probes are flooded in the same area and produce a blot of duplexes.
• C) The target sequence is part of the solid support, and the probe, which is in solution, hybridizes to the target.
• D) The probe is electrophoresed in agar and then transferred to filter paper. The antibody-based probe is then applied to the filter paper, and blots appear where there is binding.

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