What Is the Principle of the 5' Nuclease Assay (TaqMan)

question 23

Multiple Choice

What is the principle of the 5' nuclease assay (TaqMan) ?

• A) Fluorescent probe is mixed with labeled primers and a sandwich occurs where the fluorescent molecule is activated to fluorescence.
• B) The Taq DNA polymerase extends from the primers and replicates the template to which the TaqMan probe is annealed. The reporter dye is released by the 5' nuclease activity of the polymerase first and then the probe is released, thus increasing the fluorescence.
• C) The Taq DNA polymerase extends from the primers and replicates the template to which the TaqMan probe is annealed. Then the reporter dye is activated and the fluorescence increases with the number of hybrids that are produced.
• D) A bioluminescent probe is attached to the 5' side of the primer. As the Taq DNA polymerase begins adding nucleotides to the growing chain, the last nucleotide added (because of steric hindrance) will be the bioluminescent probe. Once added, the probe will begin luminescing and the concentration of the hybrids is directly proportional to the amount of luminescence that is produced.

---

Definitions:

Cataract Removal

A surgical procedure to remove the clouded lens of the eye and, in most cases, replace it with an artificial one to restore vision.

Radical Mastectomy

A surgical procedure that involves the removal of one breast, along with the underlying chest muscles and lymph nodes to treat or prevent breast cancer.

Indwelling Urinary Catheter

A catheter placed inside the bladder to allow for continuous, passive urine drainage, often used in patients unable to void naturally.

Radical Prostatectomy

A surgical procedure to remove the entire prostate gland and some of the tissue around it to treat prostate cancer.