Nested PCR Is Very Sensitive and Specific Because

question 41

Multiple Choice

Nested PCR is very sensitive and specific because

• A) it uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
• B) the assay itself basically serves as a form of internal control and ensures specificity.
• C) the assay will act as a form of external control, ensuring specificity and sensitivity.
• D) the fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

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Definitions:

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