When Choosing a Restriction Enzyme for Use in Recombinant DNA

question 34

Multiple Choice

When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?

• A)The sticky ends prevent the DNA from re- annealing to any DNA.
• B)The sticky ends make the DNA bind tighter to any cut DNA.
• C)The sticky ends stick to the purification medium making the fragments easier to purify.
• D)The sticky ends have hydrogen bonds that help re- anneal the cut DNA.
• E)The sticky ends do not have hydrogen bonds to help in re- annealing the cut DNA.

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M&M Proposition II

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