When Choosing a Restriction Enzyme for Use in Recombinant DNA

question 34

Multiple Choice

When choosing a restriction enzyme for use in recombinant DNA technologies,it is often preferred that the enzyme generate cohesive,or "sticky," ends.Why is this preferred?

• A)The sticky ends prevent the DNA from re- annealing to any DNA.
• B)The sticky ends make the DNA bind tighter to any cut DNA.
• C)The sticky ends stick to the purification medium making the fragments easier to purify.
• D)The sticky ends have hydrogen bonds that help re- anneal the cut DNA.
• E)The sticky ends do not have hydrogen bonds to help in re- annealing the cut DNA.

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Definitions:

Hypotonic Intravenous Solution

An IV solution with a lower concentration of solutes than the blood plasma, used to hydrate cells by moving water into the cell.

Sodium Chloride

Common table salt, a compound consisting of sodium and chloride ions, used in food and as an electrolyte in medical solutions.

Lactated Ringer's

A solution used in intravenous therapy to replenish body fluids, supply electrolytes, and provide a source of lactate in various medical situations.

Intravascular Hemolytic Reaction

A medical condition where red blood cells are destroyed within the blood vessels, often due to an incompatible blood transfusion.