A Scientist Is Troubleshooting the Synthesis of a CDNA Library

question 4

Multiple Choice

A scientist is troubleshooting the synthesis of a cDNA library.The scientist performs both a Northern and a Southern blot.The Northern blot demonstrated the presence of RNA while the Southern blot indicated that no cDNA was present in the sample.What is likely to be the cause of the failed synthesis of the cDNA library?

• A)proper primers
• B)temperature too cold for annealing
• C)too many dNTPs
• D)defective reverse transcriptase

---

Definitions:

Population

The entire pool from which a statistical sample is drawn and to which the results of analysis will be generalized.

Finite Population Correction Factor

The term \( \frac{(N - n)}{(N - 1)} \) that is used in the formulas for s_x and s_p whenever a finite population, rather than an infinite population, is being sampled. The generally accepted rule of thumb is to ignore the finite population correction factor whenever n/N ≤ .05.

Standard Error

The standard deviation of the sampling distribution of a statistic, most commonly of the mean.

Computing

Computing refers to the process of utilizing computer technology to complete a task or solve problems.