Sanger Sequencing Is Based on the Order in Which DdNTPs

question 40

Multiple Choice

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

• A)They have a 3' hydroxyl that allows for extension of the polynucleotide.
• B)They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
• C)They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
• D)They have a 2' hydroxyl that allows for extension of the polynucleotide.

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Definitions:

Inventory Turnover

A ratio that measures how often a company sells and replaces its stock of goods within a certain period, indicating efficiency in inventory management.

Average Inventory

The average value of a company's inventory over a specific period, calculated by adding the beginning and ending inventory and dividing by two.

Ending Inventory

The total value of goods available for sale at the end of an accounting period, calculated before the next period's beginning.

Perpetual LIFO

A method of inventory valuation that continuously updates the inventory balance using the Last In, First Out (LIFO) principle.