Sanger Sequencing Is Based on the Order in Which DdNTPs

question 40

Multiple Choice

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

• A)They have a 3' hydroxyl that allows for extension of the polynucleotide.
• B)They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
• C)They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
• D)They have a 2' hydroxyl that allows for extension of the polynucleotide.

---

Definitions:

Producer Surplus

The difference between the amount producers are willing to accept for a good or service and the actual amount they receive.

Tax

A necessary economic duty or another sort of imposition exacted from a taxpayer by a governmental agency to allocate funds for government activities and multiple public financial needs.

Good

A tangible item that satisfies some human want or need, which can be transferred from the producer to a buyer.

Deadweight Loss

A loss of economic efficiency that can occur when the equilibrium for a good or a service is not achieved, leading to a mismatch between supply and demand.