A Plasmid Contains a Multiple Cloning Site Inside the Coding

question 49

Multiple Choice

A plasmid contains a multiple cloning site inside the coding region of the lacZ gene and also contains an ampicillin resistance gene at a separate locus.When cells are transformed with a successfully recombinant plasmid containing a piece of DNA cloned into the multiple cloning site,what color will the colonies be when grown on an agar plate containing ampicillin and X- gal?

• A)blue
• B)clear
• C)white
• D)colonies will not grow
• E)green

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