Sanger Sequencing Is Based on the Order in Which DdNTPs

question 40

Multiple Choice

Sanger sequencing is based on the order in which ddNTPs are added to a growing polynucleotide.Why are ddNTPs integral to the Sanger sequencing method?

• A)They have a 3' hydroxyl that allows for extension of the polynucleotide.
• B)They do not have a 2' hydroxyl,which does not allow the extension of the polynucleotide.
• C)They do not have a 3' hydroxyl,which does not allow the extension of the polynucleotide.
• D)They have a 2' hydroxyl that allows for extension of the polynucleotide.

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