You Want to Insert Your Favorite Gene,which Has Been Digested

question 11

Multiple Choice

You want to insert your favorite gene,which has been digested with Ncol endonuclease on both ends,into a vector.Ncol recognizes the DNA sequence C*CATGG.The vector you want to use contains many endonuclease recognition sites,but does not include an Ncol site.Which restriction endonuclease would be the most suitable replacement to digest the vector?

• A)Sphl - G*CATGC
• B)Sunl - C*GTACG
• C)Kpnl - GGTAC*C
• D)CViAll - C*ATG

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Definitions:

Social Therapy

A form of therapy that emphasizes the role of social relationships in the development and recovery of mental health issues.

Medication

Substances used for medical treatment, especially for the treatment or prevention of disease.

Milieu Approach

A therapeutic method focusing on the use of social and physical environments to help individuals improve their behavior and psychological condition.

24-hour Supervision

Constant monitoring or presence of a caregiver or professional to ensure safety and provide assistance around the clock, often required for individuals with severe health conditions or disabilities.