You Want to Insert Your Favorite Gene,which Has Been Digested

question 11

Multiple Choice

You want to insert your favorite gene,which has been digested with Ncol endonuclease on both ends,into a vector.Ncol recognizes the DNA sequence C*CATGG.The vector you want to use contains many endonuclease recognition sites,but does not include an Ncol site.Which restriction endonuclease would be the most suitable replacement to digest the vector?

• A)Sphl - G*CATGC
• B)Sunl - C*GTACG
• C)Kpnl - GGTAC*C
• D)CViAll - C*ATG

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