Three Scientists Are Using Different Forms of PCR for Their

question 35

Multiple Choice

Three scientists are using different forms of PCR for their research.Abby wants to determine changes in gene expression levels in a tissue,so she uses ________.Bob is trying to extract genetic information from a wooly mammoth fossil,so he uses ________.Chris needs to express a gene in bacteria that matches the form expressed in kidney cells,so she uses ________.

• A)quantitative RT-PCR; PCR; RT-PCR
• B)PCR; quantitative RT-PCR; RT-PCR
• C)RT-PCR; PCR; quantitative RT-PCR
• D)RT-PCR; quantitative RT-PCR; PCR
• E)quantitative RT-PCR; RT-PCR; PCR

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Definitions:

Hydroxide

A negatively charged ion (OH-) consisting of oxygen and hydrogen.

Diazomethane

A highly reactive organic compound with the formula CH2N2, used in organic synthesis to introduce methyl groups or to form diazo compounds.

Carbene

A highly reactive molecule featuring a carbon atom with two non-bonded electrons and six valence electrons in total.

Carbanion

An ion that contains a negatively charged carbon atom, often a reactive intermediate in organic reactions.