You Are Studying the Function of a Recently Identified Gene

question 56

Multiple Choice

You are studying the function of a recently identified gene in C.elegans.You perform genetic screens for several months in an attempt to isolate loss-of-function gene mutations,but your efforts are unsuccessful.Your advisor suggests you try another approach to eliminate gene function.The best technique to accomplish this goal would be

• A)to design a repressor to bind to the operon of this gene.
• B)use a histone deacetylase to induce a transcriptionally inactive state.
• C)use a C.elegans strain with a homozygous TFIID mutation to prevent the translation initiation complex from forming.
• D)use RNA interference to prevent mRNA translation.

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