Your Research Project Involves the Characterization of a Recently Identified

question 32

Multiple Choice

Your research project involves the characterization of a recently identified transcription factor.As part of your project,you want to determine if this transcription factor binds directly to any of the general transcription factors.Unfortunately,you are having trouble expressing the full-length protein.What approach may help achieve your research goal?

• A)You must find a way to express the full-length protein,since transcription factor functions do not lie in separate domains.
• B)Identify the RNA that mediates the protein-protein binding.
• C)Express and purify only the DNA-binding domain,and test for binding.
• D)Express and purify only the activation domain,and test for binding.

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Definitions:

Young Women

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Enid Blyton

A prolific English author best known for her children's books, including the Famous Five and Secret Seven series.