Below Is a 2D Chromatogram That Shows the Separation of Five

question 17

Multiple Choice

Below is a 2D chromatogram that shows the separation of five amino acids.In this technique,the amino acid mixture is first separated by chromatography using a polar solvent system.Then the plate is rotated 90°,and the amino acids are further separated by electrophoresis.Identify the spots obtained from a mixture of Trp,Glu,Lys,Ile and Thr.A table with isoelectric points is included to aid in solving this problem. amino acid-pI value Trp-5.9
Glu-3.2
Lys-9.7
Ile-6.0
Thr-5.6

• A)1=Lys,2=Thr,3=Glu,4=Trp,5=Ile
• B)1=Glu,2=Thr,3=Lys,4=Trp,5=Ile
• C)1=Lys,2=Ile,3=Glu,4=Trp,5=Thr
• D)1=Glu,2=Ile,3=Lys,4=Trp,5=Thr

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