Fill in the Missing Blanks with Single Words

question 25

Essay

Fill in the missing blanks with single words.
Genome _____1______ means artificially introducing a specific change in the DNA sequence at a unique, pre-determined location within the genome of an ____2____ cell. The method relies on some form of recognition of specific sequences on both DNA strands at a locus that then allows an artificially introduced _____3____-stranded DNA break at this location. In response to the _____3____-stranded DNA break, DNA repair is carried out by the cell but after using non_____4_____ end joining DNA repair, errors can be made in the repair that can occasionally result in a desired specific DNA change. In one system genetically engineered ______5_____ ______6_____ nucleases are used in which a DNA is constructed to code for a specific sequence of ______5______ _____6______ and is then ligated to a DNA sequence that will specify a DNA ______7______ domain. A plasmid containing the resulting DNA construct can encode a ______5______ _____6______ nuclease when transfected into a cell. Using this technology, a pair of ______5______ _____6______ nucleases can be designed to bind to specific sequences on the opposite DNA strands at a desired unique position in the genome and the adjoining DNA cleavage domains work to produce the required ____3_____-strand DNA break.

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Definitions:

Limit

The maximum extent, quantity, or degree of something that is permissible or possible.

Segments

Various parts or sections into which something is divided, often used in marketing to denote different target market groups.

Pie Graph

A visual representation of data using a circular chart divided into sectors, where each sector indicates a proportion of the whole.

African Canadian

An individual of African descent who is a Canadian citizen or resident.