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In cell-based DNA cloning, a DNA population of interest (which consists of very long DNA fragments) needs to be cleaved by a _____1_____ ____2______ into manageably short DNA pieces that can be transported more easily into cells. The resulting DNA fragments are joined by a ___3____ ____4______ to a ____5____ DNA, resulting in the formation of a ____6____ ___3___. The ___5___ carries a replication origin that allows it, and the ____6____ ___3____ , to replicate within a suitable host dell, usually some type of ____7____ or ____8____ cell.


Definitions:

United States

A country located primarily in North America, consisting of 50 states, a federal district, five major self-governing territories, and various possessions.

China

A country located in East Asia, the world's most populous nation, with a rich history, dynamic economy, and significant global influence.

Child Mortality

Refers to the death of children under the age of five.

Developed Countries

Nations with a high level of economic development and standard of living, characterized by high income per capita, advanced technological infrastructure, and a wide range of services available to citizens.

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