What Is the Right Progression of Steps for a Direct-

question 27

Multiple Choice

What is the right progression of steps for a direct- sequencing experiment?

• A) Lyse cells, insert genes into plasmid, purify DNA, and amplify genes by PCR.
• B) Purify DNA, amplify genes by PCR, lyse cells, and insert genes into plasmid.
• C) Lyse cells, insert genes into plasmid, amplify genes by PCR, and purify DNA.
• D) Lyse cells, purify DNA, amplify genes by PCR, and insert genes into plasmids.

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Definitions:

Hemolysis

The destruction or rupture of red blood cells, leading to the release of hemoglobin into the blood plasma, which can occur due to various conditions or factors.

Hemostasis

The process whereby bleeding is stopped within the body, involving blood clotting and vasoconstriction.

Hematopoiesis

The process by which new blood cells are produced in the bone marrow, including red blood cells, white blood cells, and platelets.

Antigens

Substances that the immune system recognizes as foreign or dangerous, triggering an immune response.