In the Current Form of Dideoxy DNA Sequencing, the Primer

question 8

Multiple Choice

In the current form of dideoxy DNA sequencing, the primer is unlabeled and each dideoxynucleotide is labeled with a different fluorescent marker. This method

• A) eliminates the need to run sequencing reaction products on a gel.
• B) eliminates the need for sophisticated machines to read the output of each sequencing reaction.
• C) allows a DNA sequence to be determined from 4 separate sequencing reactions, each run on a separate lane of a gel.
• D) allows a DNA sequence to be determined from one sequencing reaction that is run on a single lane of a gel.

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