The Restriction Enzymes BamHI and BclI Cut at the Points

question 33

Multiple Choice

The restriction enzymes BamHI and BclI cut at the points indicated by arrows:

If one sample of DNA was cut with BamHI and another with BclI, and these two samples were mixed and treate DNA ligase, what would occur?

• A) No DNAs would be ligated joined together).
• B) Only the BamHI- cut DNA would be ligated.
• C) Only the BclI- cut DNA would be ligated.
• D) Both BamHI- cut and BclI- cut DNAs would be ligated, but only to DNA fragments cut with the same enzyme e.g., BamHI fragments would be ligated only to other BamHI fragments).
• E) Both BamHI- cut and BclI- cut DNAs would be ligated with no preference for which fragment is ligated to which other.

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