Since Dideoxy Sequencing Is Based on the Chain Termination, Why

question 23

Multiple Choice

Since dideoxy sequencing is based on the chain termination, why are normal deoxynucleotides also included in the reaction?

• A) to produce a range of DNA synthesis products that terminate at every occurrence of a particular base
• B) to provide a substrate for DNA polymerase
• C) to create DNA synthesis products long enough to allow running a gel
• D) to enhance the chain termination ability of the deoxynucleotides

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