Assume the Two E

question 30

Multiple Choice

Assume the two E.coli strains shown below are allowed to conjugate. Hfr: pro⁺, arg⁺, his⁺, lys⁺, met⁺, ampicillin- sensitive F: pro^- , arg^- , his^- , lys^- , met^- , ampicillin- resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys⁺, arg⁺, amp- resistant?

• A) ampicillin, proline, histidine, methionine
• B) ampicillin, prolein, histidine, lysine
• C) ampicillin, lysine, arginine
• D) proline, histidine, methionine
• E) lysine, arginine

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Definitions:

Skimmed Milk

A dairy product from which almost all the fat has been removed.

Physical Units Method

An accounting approach used to allocate joint costs among products based on the number of physical units at a split-off point.

Joint Costs

Costs incurred in the process of producing two or more products before the point where these products are separately identifiable.

Skimmed Milk

Milk from which the majority of the fat content has been removed, resulting in a lower calorie option.