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Membrane Fractions of Skeletal Muscle and Adipose Tissue Were Purified

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Membrane fractions of skeletal muscle and adipose tissue were purified. Before purification the samples were exposed to insulin or glucagon. The samples were then analyzed via Western blotting using antibodies against GLUT4 and GLUT2. A loading control was included in the analysis. The results are shown below. Assume that sample A is skeletal muscle. Which sample corresponds to skeletal muscle that has been exposed to insulin? Explain your choice.
Membrane fractions of skeletal muscle and adipose tissue were purified. Before purification the samples were exposed to insulin or glucagon. The samples were then analyzed via Western blotting using antibodies against GLUT4 and GLUT2. A loading control was included in the analysis. The results are shown below. Assume that sample A is skeletal muscle. Which sample corresponds to skeletal muscle that has been exposed to insulin? Explain your choice.


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