What Method Is Used to Release the O-Linked Glycan Groups $\beta$

question 49

Multiple Choice

What method is used to release the O-linked glycan groups from the membrane-associated glycoconjugates shown in 2 in the figure below?

• A) enzymatic cleavage using PNGaseF
• B) chemical cleavage using a \(\beta\)-elimination reaction
• C) chemical cleavage using a Schiff base intermediate
• D) enzymatic cleavage using \(\beta\)-galactosidase

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Definitions:

Molecular Ions

Ions composed of more than one atom, formed when a molecule gains or loses electrons.

Molecular Ion Peak

The peak in a mass spectrum that corresponds to the mass of the intact molecule, often used for molecular weight determination.

Boron Isotopes

Variants of the element boron, differing in the number of neutrons in the nucleus and having distinct nuclear properties.

Trimethylborate

A chemical compound with the formula B(OCH3)3, used as a reagent in organic synthesis and as a flame retardant.