Outline the steps used in the chain-termination method to determine the sequence of a region of DNA. Using the figure below, explain what occurs at steps A, B, C, D, and E.
Definitions:
DNA Replication
The biochemical process of producing two identical replicas from one original DNA molecule, critical for cell division and genetic inheritance.
5¢->3¢ Direction
The orientation of nucleic acid chains in DNA and RNA synthesis, indicating the direction from the 5' end (phosphate group) to the 3' end (hydroxyl group).
Replication Fork
Y-shaped structure produced during the semiconservative replication of DNA.
Template Strand
The strand of DNA that serves as the guide for synthesizing RNA during transcription, ensuring the correct sequence of nucleotides.
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