Assume That You Are Trying to Insert a Gene into a Plasmid.Someone

question 24

Multiple Choice

Assume that you are trying to insert a gene into a plasmid.Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X.The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y.You have a plasmid with a single site for Y but not for X.Your strategy should be to

• A)insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.
• B)cut the plasmid with restriction enzyme X,and insert the fragments cut with restriction enzyme Y into the plasmid.
• C)cut the DNA again with restriction enzyme Y,and insert these fragments into the plasmid cut with the same enzyme.
• D)cut the plasmid twice with restriction enzyme Y,and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.

---

Definitions:

Face Value

The nominal value printed on a financial instrument, such as a bond or stock certificate.

Zero-Coupon Bond

A bond that does not pay interest during its life but is sold at a discount from its redemption value.

Interest Paid

The amount of money paid over a period for the use of borrowed funds.

Issue Price

The price at which new or existing securities are offered for sale to the public.