A Eukaryotic Gene Has "Sticky Ends" Produced by the Restriction

question 9

Multiple Choice

A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that contain the plasmid,but not the eukaryotic gene,would grow in which of the following conditions?

• A)in the nutrient broth plus ampicillin,but not in the broth containing tetracycline
• B)only in the broth containing both antibiotics
• C)in the broth containing tetracycline,but not in the broth containing ampicillin
• D)in all four types of broth

---

Definitions:

Relationship Dynamics

Relationship dynamics in negotiations refer to the evolving interactions and power balance between parties, shaped by communication, trust, and conflict.

Formation

The process of creating or establishing something, such as a team, organization, or structure.

Stability

Stability in a strategic or organizational context refers to the state of being consistent, balanced, and not easily disrupted by external forces or internal disputes.

Allies

Allies are individuals, groups, or nations that join forces or collaborate based on shared goals, interests, or objectives, often for strategic purposes.