A Eukaryotic Gene Has "Sticky Ends" Produced by the Restriction

question 11

Multiple Choice

A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that do not take up any plasmids would grow on which media?

• A)the nutrient broth only
• B)the nutrient broth and the tetracycline broth
• C)the nutrient broth and the ampicillin broth
• D)the tetracycline broth and the ampicillin broth

---

Definitions:

Prediction Intervals

A prediction of the range within which future results are expected to occur, with a specified level of confidence, based on existing observations.

Confidence Interval

A series of values, derived from investigations of a sample, expected to incorporate the value of an unrevealed population parameter.

Population Mean

The average value of all the elements in a population, representing a central measure of tendency for the distribution of values.

Christmas Trees

In the oil and gas industry, an assembly of valves, spools, and fittings used on a wellhead to control the flow of oil and gas.