Assume That You Are Trying to Insert a Gene into a Plasmid.Someone

question 24

Multiple Choice

Assume that you are trying to insert a gene into a plasmid.Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X.The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y.You have a plasmid with a single site for Y but not for X.Your strategy should be to

• A)insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.
• B)cut the plasmid with restriction enzyme X,and insert the fragments cut with restriction enzyme Y into the plasmid.
• C)cut the DNA again with restriction enzyme Y,and insert these fragments into the plasmid cut with the same enzyme.
• D)cut the plasmid twice with restriction enzyme Y,and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.

---

Definitions:

Standard Deviation

A statistical measure that quantifies the variation or dispersion of a set of data points.

Sharpe Ratio

A measure that indicates the average return earned in excess of the risk-free rate per unit of volatility or total risk, assessing the performance of an investment.

Risk Aversion

The preference to avoid uncertainty and potential financial loss.

Holding-period Return

The total return received from holding an asset or portfolio of assets over a period of time, considering both income and capital gains.