A Eukaryotic Gene Has "Sticky Ends" Produced by the Restriction

question 9

Multiple Choice

A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that contain the plasmid,but not the eukaryotic gene,would grow in which of the following conditions?

• A)in the nutrient broth plus ampicillin,but not in the broth containing tetracycline
• B)only in the broth containing both antibiotics
• C)in the broth containing tetracycline,but not in the broth containing ampicillin
• D)in all four types of broth

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Definitions:

Emotionally Aroused

A state of heightened physiological and psychological activity and responsiveness to stimuli, often associated with intense feelings or emotions.

Persuade

The action of convincing someone to do or believe something through reasoning or argument.

Happy

A state of well-being and contentment characterized by positive emotions ranging from pleasure to intense joy.

Speisman's 1964 Study

A psychological experiment that investigated the role of cognitive appraisal in emotional experiences.