A Eukaryotic Gene Has "Sticky Ends" Produced by the Restriction

question 11

Multiple Choice

A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI.The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to the antibiotics ampicillin and tetracycline.The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene.This mixture is incubated for several hours,exposed to DNA ligase,and then added to bacteria growing in nutrient broth.The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original.Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin,nutrient broth plus tetracycline,nutrient broth plus ampicillin and tetracycline,and nutrient broth without antibiotics.
Bacteria that do not take up any plasmids would grow on which media?

• A)the nutrient broth only
• B)the nutrient broth and the tetracycline broth
• C)the nutrient broth and the ampicillin broth
• D)the tetracycline broth and the ampicillin broth

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Definitions:

Unstable Oxygen Molecules

Often referred to as free radicals, these are molecules with unpaired electrons that can cause oxidative damage to cells and tissues.

Empiricism

Empiricism is a theory that states that knowledge comes only or primarily from sensory experience.

Developmental Psychology

The scientific study of how and why human beings change over the course of their life, from infancy through old age, including cognitive, emotional, and social changes.

Comparative Psychology

Area of psychology in which the psychologists study animals and their behavior for the purpose of comparing and contrasting it to human behavior.