Your Lab Director Requests That You Add New Growth Medium

question 67

Essay

Your lab director requests that you add new growth medium to the mammalian cell cultures before heading home from the lab on a Friday night.Unfortunately, you need to make fresh medium because all the premixed bottles of medium have been used.One of the ingredients you know you need to add is a mix of the essential amino acids (those that cannot be made by the cells, but are needed in proteins).On the shelf of dry chemicals you find the amino acids you need, and you mix them into your medium, along with all the other necessary nutrients, and replace the old medium with your new medium.On Sunday, you come in to the lab just to check on your cells and find that the cells have not grown.You are sure you made the medium correctly, but on checking you see that somebody wrote a note on the dry mixture of amino acids you used: "Note: this mixture contains only ᴅ-amino acids."
A.What is the meaning of the note and how does it explain the lack of cell growth in your culture?
B.Are there any organisms that could grow using this mixture? Justify your answer.

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Definitions:

Probability

A measure of the likelihood or chance that a particular event will occur, expressed as a number between 0 and 1.

Null Hypothesis

A default hypothesis that there is no significant difference or effect, used as a starting point for statistical testing.

Region of Rejection

The range of values in a statistical test for which the null hypothesis is rejected.

Statistic

A statistic is a numerical measurement describing some characteristic of a sample, which is a subset of the population data.