You Are Studying a Biochemical Pathway That Requires ATP as an Energy

question 22

Multiple Choice

You are studying a biochemical pathway that requires ATP as an energy source.To your dismay, the reactions soon stop, partly because the ATP is rapidly used up and partly because an excess of ADP builds up and inhibits the enzymes involved.You are about to give up when the following table from a biochemistry textbook catches your eye. $\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\text {hydrolysis}$
$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\underline{\text {reaction}}$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$$\quad$ $\Delta G^{\circ}$
$\text { creatine + ATP } \quad\quad\quad\quad \stackrel{\text { enzyme A }}{\longrightarrow} \text { creatine phosphate + ADP +3 kcal/mole }$

$\text { ATP }+\mathrm{H}_{2} \mathrm{O} \quad\quad\quad\quad\quad \stackrel{\text { enzyme B }}{\longrightarrow} \mathrm{ADP}+\text { phosphate } \quad \quad\quad\quad-7.3 \mathrm{kcal} / \text { mole }$

$\text { pyrophosphate }+\mathrm{H}_{2} \mathrm{O} \quad \stackrel{\text { enzyme } \mathrm{C}}{\longrightarrow} 2 \text { phosphate } \quad\quad\quad\quad \quad\quad\quad\quad-7 \mathrm{kcal} / \text { mole }$

glucose 6-phosphate $+\mathrm{H}_{2} \mathrm{O} \quad\stackrel{\text { enzyme D }}{\longrightarrow}$ qlucose + phosphate $\quad\quad\quad\quad\quad\quad\quad-3.3 \mathrm{kcal} / \mathrm{mole}$
Table 3-43
Which of the following reagents is/are most likely to revitalize your reaction?

• A)a vast excess of ATP
• B)glucose 6-phosphate and enzyme D
• C)creatine phosphate and enzyme A
• D)pyrophosphate

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