Protein E Can Bind to Two Different Proteins, S and I.The

question 46

Multiple Choice

Protein E can bind to two different proteins, S and I.The binding reactions are described by the following equations and values:
E + S → ES K_eq for ES = 10
E + I → EI K_eq for EI = 2
Given the equilibrium constant values, which one of the following statements is TRUE?

• A)E binds I more tightly than S.
• B)When S is present in excess, no I molecules will bind to E.
• C)The binding energy of the ES interaction is greater than that of the EI interaction.
• D)Changing an amino acid on the binding surface of I from a basic amino acid to an acidic one will probably make the free energy of association with E more negative.

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