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Consider a description of an enzymatic reaction pathway that begins with the binding of substrate S to enzyme E, and ends with the release of product P from the enzyme.
E + S → ES → EP → E + P
In many circumstances,
Km = [E][S]/[ES]
A.What proportion of enzyme molecules is bound to substrate when [S] = Km?
B.Recall that when [S] = Km, the reaction rate is ½Vmax.Does your answer to part A make sense in the light of this rate information?
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