To Study How Proteins Fold, Scientists Must Be Able to Purify

question 60

Multiple Choice

To study how proteins fold, scientists must be able to purify the protein of interest, use solvents to denature the folded protein, and observe the process of refolding at successive time points.What is the effect of the solvents used in the denaturation process?

• A)The solvents break all covalent interactions.
• B)The solvents break all noncovalent interactions.
• C)The solvents break some of the noncovalent interactions, resulting in a misfolded protein.
• D)The solvents create a new protein conformation.

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Definitions:

Nucleus

A membrane-bound organelle within a cell that contains most of the cell's genetic material and regulates gene expression.

Cell

The basic structural, functional, and biological unit of all living organisms, consisting of cytoplasm enclosed within a membrane.

Cytoskeleton

A network of fibers within cells that provides structure, shape, and aids in movement.

Microtubules

Cylindrical structures within the cytoplasm of eukaryotic cells that are involved in cellular processes like division, growth, and transport.